EF1a-TBET-T2A-mCHERRY
(Plasmid
#224296)
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PurposeExpresses mouse TBET and mCherry under EF1a promoter
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 224296 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneMSCV
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Vector typeMammalian Expression, Retroviral
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Selectable markersmCherry
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameTBET T2A mCherry
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SpeciesM. musculus (mouse)
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Entrez GeneTbx21 (a.k.a. TBT1, Tbet, Tblym)
- Promoter EF1a
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer ccatgGGCATCGTGGAGCCG (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
EF1a-TBET-T2A-mCHERRY was a gift from Nikhil Joshi (Addgene plasmid # 224296 ; http://n2t.net/addgene:224296 ; RRID:Addgene_224296) -
For your References section:
KLF2 maintains lineage fidelity and suppresses CD8 T cell exhaustion during acute LCMV infection. Fagerberg E, Attanasio J, Dien C, Singh J, Kessler EA, Abdullah L, Shen J, Hunt BG, Connolly KA, De Brouwer E, He J, Iyer NR, Buck J, Borr ER, Damo M, Foster GG, Giles JR, Huang YH, Tsang JS, Krishnaswamy S, Cui W, Joshi NS. Science. 2025 Jan 2;387(6735):eadn2337. doi: 10.1126/science.adn2337. Epub 2025 Feb 14. 10.1126/science.adn2337 PubMed 39946463