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Addgene

U6-PGK-hCD2
(Plasmid #224294)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 224294 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    MSCV
  • Vector type
    Mammalian Expression, Retroviral, CRISPR
  • Selectable markers
    CD2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    U6 sgRNA cassette with CD2 under PGK promoter
  • gRNA/shRNA sequence
    empty
  • Species
    H. sapiens (human)
  • Entrez Gene
    CD2 (a.k.a. LFA-2, SRBC, T11)
  • Promoter U6/PGK

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    U6-PGK-hCD2 was a gift from Nikhil Joshi (Addgene plasmid # 224294 ; http://n2t.net/addgene:224294 ; RRID:Addgene_224294)

  • For your References section:

    KLF2 maintains lineage fidelity and suppresses CD8 T cell exhaustion during acute LCMV infection. Fagerberg E, Attanasio J, Dien C, Singh J, Kessler EA, Abdullah L, Shen J, Hunt BG, Connolly KA, De Brouwer E, He J, Iyer NR, Buck J, Borr ER, Damo M, Foster GG, Giles JR, Huang YH, Tsang JS, Krishnaswamy S, Cui W, Joshi NS. Science. 2025 Jan 2;387(6735):eadn2337. doi: 10.1126/science.adn2337. Epub 2025 Feb 14. 10.1126/science.adn2337 PubMed 39946463
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