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Addgene

optoFcR
(Plasmid #224291)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 224291 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pHR
  • Total vector size (bp) 11362
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Fc receptor gamma
  • Alt name
    Intracellular domain of FcgammaR
  • Species
    M. musculus (mouse)
  • Mutation
    aa 45-86 only
  • Entrez Gene
    Fcer1g (a.k.a. CD23, FcR-gamma, FcR[g], FcRgamma, Fce1g, FcepsilonRI, Ly-50)
  • Promoter SFFV
  • Tags / Fusion Proteins
    • myristoylation sequence (N terminal on insert)
    • mScarlet-Cry2-olig (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2023.11.14.567059 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    optoFcR was a gift from Meghan Morrissey (Addgene plasmid # 224291 ; http://n2t.net/addgene:224291 ; RRID:Addgene_224291)

  • For your References section:

    Prior Fc receptor activation primes macrophages for increased sensitivity to IgG via long-term and short-term mechanisms. Bond A, Fiaz S, Rollins K, Nario JEQ, Snyder ET, Atkins DJ, Rosen SJ, Granados A, Dey SS, Wilson MZ, Morrissey MA. Dev Cell. 2024 Aug 9:S1534-5807(24)00457-X. doi: 10.1016/j.devcel.2024.07.017. 10.1016/j.devcel.2024.07.017 PubMed 39137774
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