pcDNA3.1(+) R-Ras1(G38C)
(Plasmid
#224286)
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PurposeCell transfection and expression of R-Ras1 (G38C)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 224286 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.1(+)
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameR-Ras1
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SpeciesH. sapiens (human)
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MutationG38C
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Entrez GeneRRAS (a.k.a. R-Ras)
Cloning Information
- Cloning method Gene Synthesis
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3.1(+) R-Ras1(G38C) was a gift from Kevan Shokat (Addgene plasmid # 224286 ; http://n2t.net/addgene:224286 ; RRID:Addgene_224286) -
For your References section:
Targeting Ras-, Rho-, and Rab-family GTPases via a conserved cryptic pocket. Morstein J, Bowcut V, Fernando M, Yang Y, Zhu L, Jenkins ML, Evans JT, Guiley KZ, Peacock DM, Krahnke S, Lin Z, Taran KA, Huang BJ, Stephen AG, Burke JE, Lightstone FC, Shokat KM. Cell. 2024 Sep 6:S0092-8674(24)00908-5. doi: 10.1016/j.cell.2024.08.017. 10.1016/j.cell.2024.08.017 PubMed 39255801