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Addgene

pcDNA3.1(+) EGFP-Rac1(G12C, K96H, P29S)
(Plasmid #224282)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 224282 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pcDNA3.1(+)
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Rac1
  • Species
    H. sapiens (human)
  • Mutation
    G12C, K96H, P29S
  • Entrez Gene
    RAC1 (a.k.a. MIG5, MRD48, Rac-1, TC-25, p21-Rac1)

Cloning Information

  • Cloning method Gene Synthesis

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1(+) EGFP-Rac1(G12C, K96H, P29S) was a gift from Kevan Shokat (Addgene plasmid # 224282 ; http://n2t.net/addgene:224282 ; RRID:Addgene_224282)
  • For your References section:

    Targeting Ras-, Rho-, and Rab-family GTPases via a conserved cryptic pocket. Morstein J, Bowcut V, Fernando M, Yang Y, Zhu L, Jenkins ML, Evans JT, Guiley KZ, Peacock DM, Krahnke S, Lin Z, Taran KA, Huang BJ, Stephen AG, Burke JE, Lightstone FC, Shokat KM. Cell. 2024 Sep 6:S0092-8674(24)00908-5. doi: 10.1016/j.cell.2024.08.017. 10.1016/j.cell.2024.08.017 PubMed 39255801