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pGEX-GST-BcPC-PLC
(Plasmid #224255)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 224255 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEX-6P-1
  • Backbone manufacturer
    Cytiva
  • Backbone size w/o insert (bp) 4265
  • Total vector size (bp) 5117
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Bacillus cereus phospholipase C
  • Alt name
    Phosphatidylcholine-specific phospholipase C
  • Alt name
    PC-PLC
  • Alt name
    Phospholipase C, EC:3.1.4.3,
  • Species
    Bacillus cereus ATCC 14579
  • Insert Size (bp)
    852
  • Mutation
    The gene of Bacillus cereus ATCC 14579 was used for cloning
  • GenBank ID
    Q81HW1 · Q81HW1_BACCR WP_000730997.1
  • Entrez Gene
    plC (a.k.a. C2I25_RS08115, C2I25_08115)
  • Entrez Gene
    plC (a.k.a. FOC91_RS06050, FOC91_06050)
  • Promoter lac promoter
  • Tags / Fusion Proteins
    • GST (N terminal on backbone)
    • HRV-3C (LEVLFQGP) site (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer CATTAGGCACCCCAGGCTTTACACTTTATG
  • 3′ sequencing primer GCTTACAGACAAGCTGTGACCGTC
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX-GST-BcPC-PLC was a gift from Chiaki Murakami (Addgene plasmid # 224255 ; http://n2t.net/addgene:224255 ; RRID:Addgene_224255)

  • For your References section:

    Development of a liquid chromatography-mass spectrometry based enzyme activity assay for phosphatidylcholine-specific phospholipase C. Murakami C, Mizuno S, Kado S, Sakane F. Anal Biochem. 2017 Jun 1;526:43-49. doi: 10.1016/j.ab.2017.03.010. Epub 2017 Mar 14. 10.1016/j.ab.2017.03.010 PubMed 28315318
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