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pDest17_hnRNPA1_YtoW
(Plasmid #224253)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 224253 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDest17
  • Backbone manufacturer
    Invitrogen
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    hnRNPA1_YtoW
  • Species
    H. sapiens (human)
  • Mutation
    Y52W, Y59W, Y75W, Y81W, Y110W, Y120W, Y129W
  • Entrez Gene
    HNRNPA1 (a.k.a. ALS19, ALS20, HNRPA1, HNRPA1L3, IBMPFD3, MPD3, UP 1, hnRNP A1, hnRNP-A1)
  • Promoter T7
  • Tag / Fusion Protein
    • 6xHis (N terminal on insert)

Cloning Information

  • Cloning method Gateway Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Contains TEV cleavage site.

Please visit https://pubmed.ncbi.nlm.nih.gov/37066350/ for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDest17_hnRNPA1_YtoW was a gift from Tanja Mittag (Addgene plasmid # 224253 ; http://n2t.net/addgene:224253 ; RRID:Addgene_224253)

  • For your References section:

    Sequence-specific interactions determine viscoelasticity and aging dynamics of protein condensates. Alshareedah I, Borcherds WM, Cohen SR, Singh A, Posey AE, Farag M, Bremer A, Strout GW, Tomares DT, Pappu RV, Mittag T, Banerjee PR. bioRxiv [Preprint]. 2023 Dec 26:2023.04.06.535902. doi: 10.1101/2023.04.06.535902. 10.1101/2023.04.06.535902 PubMed 37066350
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