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pJExpress401-KDAC4 CD H976Y
(Plasmid #224250)

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Item Catalog # Description Quantity Price (USD)
Plasmid 224250 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pJExpress401
  • Backbone manufacturer
    DNA 2.0
  • Backbone size w/o insert (bp) 3936
  • Total vector size (bp) 5292
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    KDAC4
  • Alt name
    HDAC4
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1356
  • Mutation
    Only includes residues 648-1083 (catalytic domain). Histidine 976 mutated to tyrosine.
  • Entrez Gene
    HDAC4 (a.k.a. AHO3, BDMR, HA6116, HD4, HDAC-4, HDAC-A, HDACA, NEDCHF, NEDCHID)
  • Promoter T5
  • Tag / Fusion Protein
    • TEV-cleavable His6 (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer CTCGAAAATAATAAAGGGAAAATCAG
  • 3′ sequencing primer TGGTAGTGTGGGGACTC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Codon optimized for bacterial expression. Catalytic domain only. Has enhanced activity with acetylated peptides compared to wild-type.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJExpress401-KDAC4 CD H976Y was a gift from Terry Watt (Addgene plasmid # 224250 ; http://n2t.net/addgene:224250 ; RRID:Addgene_224250)

  • For your References section:

    Lysine Deacetylases Exhibit Distinct Changes in Activity Profiles Due to Fluorophore Conjugation of Substrates. Toro TB, Bryant JR, Watt TJ. Biochemistry. 2017 Aug 29;56(34):4549-4558. doi: 10.1021/acs.biochem.7b00270. Epub 2017 Aug 16. 10.1021/acs.biochem.7b00270 PubMed 28749131
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