pET11a_PTK2b_Kinase
(Plasmid
#224242)
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PurposeExpression of human PTK2b Kinase domain in E. coli
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 224242 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET11a
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Backbone manufacturerGenscript
- Backbone size w/o insert (bp) 5644
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameptk2b_kinase
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Alt nameCADTK, CAKB, FADK2, FAK2, PKB, PTK, PYK2, RAFTK
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SpeciesH. sapiens (human)
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Insert Size (bp)900
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Entrez GenePTK2B (a.k.a. CADTK, CAKB, FADK2, FAK2, PKB, PTK, PYK2, RAFTK)
- Promoter T7 promotor
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Tag
/ Fusion Protein
- 6xHis-TEV (N terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer T7, M13_fw
- 3′ sequencing primer M13_ter (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byGenscript
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET11a_PTK2b_Kinase was a gift from Andrew Mesecar (Addgene plasmid # 224242 ; http://n2t.net/addgene:224242 ; RRID:Addgene_224242)