pET28a_LYN
(Plasmid
#224238)
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PurposeExpression of human LYN Kinase in E. coli
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 224238 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET28a
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Backbone manufacturerGenscript
- Backbone size w/o insert (bp) 5366
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namelyn
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Alt nameJTK8; SAIDV; p53Lyn; p56Lyn
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SpeciesH. sapiens (human)
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Insert Size (bp)1455
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Entrez GeneLYN (a.k.a. JTK8, SAIDV, p53Lyn, p56Lyn)
- Promoter T7 promotor
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Tags
/ Fusion Proteins
- 6xHis-TEV (N terminal on insert)
- 6xHis (C terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer T7, M13_fw
- 3′ sequencing primer M13_ter (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byGenscript
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET28a_LYN was a gift from Andrew Mesecar (Addgene plasmid # 224238 ; http://n2t.net/addgene:224238 ; RRID:Addgene_224238)