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Addgene

pJExpress401-KDAC8
(Plasmid #224208)

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Item Catalog # Description Quantity Price (USD)
Plasmid 224208 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pJExpress401
  • Backbone manufacturer
    DNA 2.0
  • Backbone size w/o insert (bp) 3936
  • Total vector size (bp) 5109
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    KDAC8
  • Alt name
    HDAC8
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1173
  • Entrez Gene
    HDAC8 (a.k.a. CDA07, CDLS5, HD8, HDACL1, KDAC8, MRXS6, RPD3, WTS)
  • Promoter T5
  • Tag / Fusion Protein
    • TEV-cleavable His6 (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer CTCGAAAATAATAAAGGGAAAATCAG
  • 3′ sequencing primer TGGTAGTGTGGGGACTC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Codon optimized for bacterial expression.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJExpress401-KDAC8 was a gift from Terry Watt (Addgene plasmid # 224208 ; http://n2t.net/addgene:224208 ; RRID:Addgene_224208)

  • For your References section:

    KDAC8 substrate specificity quantified by a biologically relevant, label-free deacetylation assay. Toro TB, Watt TJ. Protein Sci. 2015 Dec;24(12):2020-32. doi: 10.1002/pro.2813. Epub 2015 Oct 7. 10.1002/pro.2813 PubMed 26402585
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