pBABE-puro mCherry-EGFP-LC3B
(Plasmid #22418)

• Purpose: retroviral expression of mCherry, EGFP-tagged LC3
• Depositing Lab: Jayanta Debnath
• Publication: N'Diaye et al EMBO Rep. 2009 Feb;10(2):173-9. doi: 10.1038/embor.2008.238. Epub 2009 Jan 16.

Backbone

• Vector backbone: pBABE-puro
• Backbone size w/o insert (bp): 5169
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: microtubule-associated protein 1 light chain 3 beta
• Alt name: MAP1LC3B
• Alt name: LC3B
• Alt name: LC3
• Species: H. sapiens (human)
• Insert Size (bp): 1900
• GenBank ID: BC045759
• Entrez Gene: MAP1LC3B (a.k.a. ATG8F, LC3B, MAP1A/1BLC3, MAP1LC3B-a)
• Tags / Fusion Proteins:
  • mCherry (N terminal on insert)
  • eGFP (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (destroyed during cloning)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: n/a

Resource Information

• Addgene Notes:
  • Addgene diagnostic digest
  • Sequence and user feedback from Dr. Ian Gentle,
• A portion of this plasmid was derived from a plasmid made by: pDest-mCherry-EGFP-hLC3b from Terje Johansen from the Biochemistry Department, Institute of Medical Biology, University of Tromsøl.
• Articles Citing this Plasmid:
  • 193 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please click the "Notes from Addgene" link above for sequence submitted by a requesting scientist and further observations on using this plasmid.