AiP12442 - pAAV-AiE0612h-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2442)
(Plasmid
#224162)
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PurposeAiE0612h is an enhancer sequence, designed to drive AAV-mediated transgene expression in striatal D2 MSN>D1 MSN
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Depositing Labs
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 224162 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonerAAV
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Vector typeAAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSYFP2
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SpeciesSynthetic
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Allen Institute plasmid alias: CN2442 . This work supported by NIH BRAIN Initiative grant UF1MH128339.
There is a 1bp deletion in AiE0612h, depositor confirmed this does not impact plasmid function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
AiP12442 - pAAV-AiE0612h-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2442) was a gift from The Allen Institute for Brain Science & Jonathan Ting (Addgene plasmid # 224162 ; http://n2t.net/addgene:224162 ; RRID:Addgene_224162)