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Addgene

pCS2vegf121
(Plasmid #22416)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 22416 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pCS2+
  • Backbone size w/o insert (bp) 4000
  • Vector type
    zebrafish expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    vegf
  • Alt name
    vegfaa
  • Species
    D. rerio (zebrafish)
  • Entrez Gene
    vegfaa (a.k.a. vegf, vegfa, wu:fj82c06)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (unknown if destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)
  • 5′ sequencing primer CMV-F
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCS2vegf121 was a gift from Nathan Lawson (Addgene plasmid # 22416 ; http://n2t.net/addgene:22416 ; RRID:Addgene_22416)
  • For your References section:

    sonic hedgehog and vascular endothelial growth factor act upstream of the Notch pathway during arterial endothelial differentiation. Lawson ND, Vogel AM, Weinstein BM. Dev Cell. 2002 Jul . 3(1):127-36. 10.1016/S1534-5807(02)00198-3 PubMed 12110173