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Addgene

GSPD1_R252L
(Plasmid #224153)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 224153 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET303/CT-His
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    GSPD-1(R252L)
  • Species
    C. elegans (nematode)
  • Mutation
    R252L
  • Tag / Fusion Protein
    • LeuGlu-6xHis (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GSPD1_R252L was a gift from Katherine Walstrom (Addgene plasmid # 224153 ; http://n2t.net/addgene:224153 ; RRID:Addgene_224153)

  • For your References section:

    Modeling human glucose-6-phosphate dehydrogenase mutations using C. elegans GSPD-1. Loges LN, Walstrom KM. MicroPubl Biol. 2021 Sep 10;2021:10.17912/micropub.biology.000451. doi: 10.17912/micropub.biology.000451. eCollection 2021. 10.17912/micropub.biology.000451 PubMed 34532700
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