GSPD1_AJ
(Plasmid
#224152)
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PurposeIPTG-inducible expression of C. elegans protein GSPD-1 with a His tag
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 224152 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET303/CT-His
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameGSPD-1
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SpeciesC. elegans (nematode)
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Entrez GeneB0035.5
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Tag
/ Fusion Protein
- LeuGlu-6xHis (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
GSPD1_AJ was a gift from Katherine Walstrom (Addgene plasmid # 224152 ; http://n2t.net/addgene:224152 ; RRID:Addgene_224152) -
For your References section:
Modeling human glucose-6-phosphate dehydrogenase mutations using C. elegans GSPD-1. Loges LN, Walstrom KM. MicroPubl Biol. 2021 Sep 10;2021:10.17912/micropub.biology.000451. doi: 10.17912/micropub.biology.000451. eCollection 2021. 10.17912/micropub.biology.000451 PubMed 34532700