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Addgene

MDH2_EC
(Plasmid #224146)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 224146 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBAD/HisA
  • Backbone manufacturer
    Invitrogen
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    MDH-2
  • Species
    C. elegans (nematode)
  • Entrez Gene
    mdh-2 (a.k.a. CELE_F20H11.3)
  • Tag / Fusion Protein
    • His tag, Xpress tag, enterokinase cleaveage site (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MDH2_EC was a gift from Katherine Walstrom (Addgene plasmid # 224146 ; http://n2t.net/addgene:224146 ; RRID:Addgene_224146)

  • For your References section:

    Kinetic characterization and thermostability of C. elegans cytoplasmic and mitochondrial malate dehydrogenases. Thomas MJ, Cassidy ER, Robinson DS, Walstrom KM. Biochim Biophys Acta Proteins Proteom. 2022 Jan;1870(1):140722. doi: 10.1016/j.bbapap.2021.140722. Epub 2021 Oct 4. 10.1016/j.bbapap.2021.140722 PubMed 34619358
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