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Addgene

MDH1_KW
(Plasmid #224145)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 224145 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTXB1
  • Backbone manufacturer
    NEB
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    MDH-1
  • Species
    C. elegans (nematode)
  • Entrez Gene
    F46E10.10
  • Tag / Fusion Protein
    • Ala-intein-CBD (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MDH1_KW was a gift from Katherine Walstrom (Addgene plasmid # 224145 ; http://n2t.net/addgene:224145 ; RRID:Addgene_224145)

  • For your References section:

    Kinetic characterization and thermostability of C. elegans cytoplasmic and mitochondrial malate dehydrogenases. Thomas MJ, Cassidy ER, Robinson DS, Walstrom KM. Biochim Biophys Acta Proteins Proteom. 2022 Jan;1870(1):140722. doi: 10.1016/j.bbapap.2021.140722. Epub 2021 Oct 4. 10.1016/j.bbapap.2021.140722 PubMed 34619358
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