GLB10_MS
(Plasmid
#224139)
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PurposeIPTG-inducible expression of C. elegans protein GLB-10 with a His tag
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 224139 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET303/CT-His
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameGLB-10
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SpeciesC. elegans (nematode)
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Tag
/ Fusion Protein
- LeuGlu-6xHis (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer T7 (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
GLB10_MS was a gift from Katherine Walstrom (Addgene plasmid # 224139 ; http://n2t.net/addgene:224139 ; RRID:Addgene_224139) -
For your References section:
UV-Visible Heme Absorbance Spectroscopic Characterization of Caenorhabditis elegans Globin Proteins GLB-10 and GLB-31. McAllister A, Walstrom K. Mendeley Data. 2025 Jan 2; V1. doi: 10.17632/td8sy4xfmp.1 10.17632/td8sy4xfmp.1