pAAV2-CAGIG-WGA-mCherry
(Plasmid #224119)

• Purpose: To express the fusion protein composed of the Wheat germ agluttinin to the fluorescent protein mCherry. Labels the Golgi apparatus, ER and the secretory pathway. Secreted form, labels Lysosomes.
• Depositing Lab: Pantelis Tsoulfas
• Publication: Actin-Endosomes (unpublished)

Backbone

• Vector backbone: pAAV2-CAGIG
• Backbone size w/o insert (bp): 4726
• Total vector size (bp): 6070
• Vector type: Mammalian Expression, AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: WGA-mCherry
• Alt name: Wheat germ Isolectin 2, WGA2
• Species: Triticum aestivum
• Insert Size (bp): 1344
• Mutation: All mutations related to the WGA in this construct can be found in PMIDs: 1483481, 10027287, 35524141
• GenBank ID: NP_001414927 NP_001414927
• Promoter: CAGIG
• Tags / Fusion Proteins:
  • WGA (N terminal on insert)
  • mCherry (C terminal on insert)

Cloning Information

• Cloning method: Gene Synthesis
• 5′ sequencing primer: pACCS-5
• 3′ sequencing primer: WPRE-R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry