pAAV2-CAGIG-WGA-mCherry
(Plasmid
#224119)
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PurposeTo express the fusion protein composed of the Wheat germ agluttinin to the fluorescent protein mCherry. Labels the Golgi apparatus, ER and the secretory pathway. Secreted form, labels Lysosomes.
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 224119 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepAAV2-CAGIG
- Backbone size w/o insert (bp) 4726
- Total vector size (bp) 6070
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Vector typeMammalian Expression, AAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameWGA-mCherry
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Alt nameWheat germ Isolectin 2, WGA2
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SpeciesTriticum aestivum
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Insert Size (bp)1344
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MutationAll mutations related to the WGA in this construct can be found in PMIDs: 1483481, 10027287, 35524141
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GenBank IDNP_001414927 NP_001414927
- Promoter CAGIG
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Tags
/ Fusion Proteins
- WGA (N terminal on insert)
- mCherry (C terminal on insert)
Cloning Information
- Cloning method Gene Synthesis
- 5′ sequencing primer pACCS-5
- 3′ sequencing primer WPRE-R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV2-CAGIG-WGA-mCherry was a gift from Pantelis Tsoulfas (Addgene plasmid # 224119 ; http://n2t.net/addgene:224119 ; RRID:Addgene_224119)
Map uploaded by the depositor.
