pSDC803_IKZF1_mutant
(Plasmid
#224113)
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PurposeInsect cell expression vector for IKZF1 mutant for CRBN cryoEM pre-blotting
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 224113 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAC8
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Vector typeInsect Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameIKZF1
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SpeciesH. sapiens (human)
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Insert Size (bp)300
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MutationResidues 140-196, Q146A and G151N
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Entrez GeneIKZF1 (a.k.a. CVID13, Hs.54452, IK1, IKAROS, LYF1, LyF-1, PPP1R92, PRO0758, ZNFN1A1)
- Promoter polyhedrin
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Tag
/ Fusion Protein
- Flag-TEV (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NotI (not destroyed)
- 3′ cloning site KpnI (not destroyed)
- 5′ sequencing primer - (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSDC803_IKZF1_mutant was a gift from Eric Fischer (Addgene plasmid # 224113 ; http://n2t.net/addgene:224113 ; RRID:Addgene_224113) -
For your References section:
Continuous evolution of compact protein degradation tags regulated by selective molecular glues. Mercer JAM, DeCarlo SJ, Roy Burman SS, Sreekanth V, Nelson AT, Hunkeler M, Chen PJ, Donovan KA, Kokkonda P, Tiwari PK, Shoba VM, Deb A, Choudhary A, Fischer ES, Liu DR. Science. 2024 Mar 15;383(6688):eadk4422. doi: 10.1126/science.adk4422. Epub 2024 Mar 15. 10.1126/science.adk4422 PubMed 38484051