pFA6a-link-ymScarletI3-SpHis5
(Plasmid
#224076)
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PurposeYeast tagging vector to create a gene fusion with yeast optimized mScarletI3 with SpHis5 selection
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 224076 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepFA6A
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Vector typeYeast Expression
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Selectable markersHIS5
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameymScarletI3
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SpeciesSynthetic
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Tag
/ Fusion Protein
- ymScarletI3
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pFA6a-link-ymScarletI3-SpHis5 was a gift from Bas Teusink (Addgene plasmid # 224076 ; http://n2t.net/addgene:224076 ; RRID:Addgene_224076)