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TLNRD1(4H)-2E_pET151
(Plasmid #224068)

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Item Catalog # Description Quantity Price (USD)
Plasmid 224068 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pET151/D-TOPO
  • Backbone size w/o insert (bp) 5760
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    tlnrd1
  • Alt name
    mesdc1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    393
  • Mutation
    K192E/R233E double mutant; prevents actin binding and CCM2 binding
  • Entrez Gene
    TLNRD1 (a.k.a. MESDC1)
  • Promoter T7
  • Tag / Fusion Protein
    • 6xHIS; V5 (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    TLNRD1(4H)-2E_pET151 was a gift from Ben Goult (Addgene plasmid # 224068 ; http://n2t.net/addgene:224068 ; RRID:Addgene_224068)

  • For your References section:

    TLNRD1 is a CCM complex component and regulates endothelial barrier integrity. Ball NJ, Ghimire S, Follain G, Pajari AO, Wurzinger D, Vaitkeviciute M, Cowell AR, Berki B, Ivaska J, Paatero I, Goult BT, Jacquemet G. J Cell Biol. 2024 Sep 2;223(9):e202310030. doi: 10.1083/jcb.202310030. Epub 2024 Jul 16. 10.1083/jcb.202310030 PubMed 39013281
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