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TLNRD1(4H)_pEGFP-N3
(Plasmid #224067)

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Item Catalog # Description Quantity Price (USD)
Plasmid 224067 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pEGFP-N3
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    tlnrd1
  • Alt name
    mesdc1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    393
  • Entrez Gene
    TLNRD1 (a.k.a. MESDC1)
  • Promoter CMV
  • Tag / Fusion Protein
    • EGFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer EGFP-N
  • 3′ sequencing primer SV40pA-R
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    TLNRD1(4H)_pEGFP-N3 was a gift from Ben Goult (Addgene plasmid # 224067 ; http://n2t.net/addgene:224067 ; RRID:Addgene_224067)

  • For your References section:

    TLNRD1 is a CCM complex component and regulates endothelial barrier integrity. Ball NJ, Ghimire S, Follain G, Pajari AO, Wurzinger D, Vaitkeviciute M, Cowell AR, Berki B, Ivaska J, Paatero I, Goult BT, Jacquemet G. J Cell Biol. 2024 Sep 2;223(9):e202310030. doi: 10.1083/jcb.202310030. Epub 2024 Jul 16. 10.1083/jcb.202310030 PubMed 39013281
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