CCM2(413-438)_pHisSUMO(K0)
(Plasmid #224065)

• Purpose: Bacterial expression of the C-terminal helix of CCM2 (residues 413-438) fused to an N-terminal hexa-histidine tag and SUMO(K0) [all lysines in SUMO mutated to arginines]. HRV-3C cleavage site
• Depositing Lab: Ben Goult
• Publication: Ball et al J Cell Biol. 2024 Sep 2;223(9):e202310030. doi: 10.1083/jcb.202310030. Epub 2024 Jul 16.

Backbone

• Vector backbone: pET47b
• Backbone size w/o insert (bp): 5484
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: ccm2
• Species: H. sapiens (human)
• Insert Size (bp): 78
• Entrez Gene: CCM2 (a.k.a. C7orf22, OSM, PP10187)
• Promoter: T7
• Tag / Fusion Protein:
  • 6xHIS; SUMO(K0) (N terminal on insert)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: T7 Fwd
• 3′ sequencing primer: T7 Rev

Resource Information

• Supplemental Documents:
  • CCM2(413-438)_pHisSUMO(K0).gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA