pAc5.1B-lambdaN-HA-DmGW182
(Plasmid
#22406)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 22406 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAc5.1B-lambdaN
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Backbone manufacturerElisa Izaurralde
- Backbone size w/o insert (bp) 5459
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Vector typeDrosophila expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsDh5 alpha and XL1-blue
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namegawky, variant A
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Alt nameGW182
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SpeciesD. melanogaster (fly)
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Insert Size (bp)4155
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GenBank IDNM_166780
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Entrez Genegw (a.k.a. Dmel_CG31992, CG11484, CG31992, CG9905, Dmel\CG31992, GQ182, GW, GW182, Gw, Gw182, anon-EST:Posey205, dGW182, gw182)
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Tag
/ Fusion Protein
- lambdaN-HA (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer Ac5 forward primer site
- 3′ sequencing primer BGH reverse priming site (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAc5.1B-lambdaN-HA-DmGW182 was a gift from Elisa Izaurralde (Addgene plasmid # 22406 ; http://n2t.net/addgene:22406 ; RRID:Addgene_22406) -
For your References section:
mRNA degradation by miRNAs and GW182 requires both CCR4:NOT deadenylase and DCP1:DCP2 decapping complexes. Behm-Ansmant I, Rehwinkel J, Doerks T, Stark A, Bork P, Izaurralde E. Genes Dev. 2006 Jul 15. 20(14):1885-98. 10.1101/gad.1424106 PubMed 16815998