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Addgene

pBABEpuro GFP-LC3 Notes

Addgene diagnostic digest

This gel depicts the NEB 1kb ladder and a digestion of the plasmid with the following enzymes: SalI and SalI/SnaBI

We expected the following band sizes for this digest: ~7.5kb for linearization with SalI, then 1.5kb and 5kb bands for the double. We see the expected pattern.