pBABEpuro GFP-LC3
(Plasmid #22405)

• Purpose: retroviral expression of GFP-tagged LC3
• Depositing Lab: Jayanta Debnath
• Publication: Fung et al Mol Biol Cell. 2008 Mar . 19(3):797-806.

Backbone

• Vector backbone: pBABE-puro
• Backbone manufacturer: Addgene plasmid # 1764
• Backbone size w/o insert (bp): 5169
• Modifications to backbone: GFP-LC3 was subcloned from pEGFPC1-LC3 into the SnaB1 and Sal1 sites of pBABEpuro
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: microtubule-associated protein 1 light chain 3 beta
• Alt name: Map1lc3b
• Alt name: LC3b
• Alt name: LC3
• Species: R. norvegicus (rat)
• Insert Size (bp): 1500
• GenBank ID: U05784
• Entrez Gene: Map1lc3b (a.k.a. LC3B, Map1lc3, Mpl3, zbs559)
• Tag / Fusion Protein:
  • GFP (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SnaBI (not destroyed)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: pBABE-5
• 3′ sequencing primer: pBABE-3

Resource Information

• Addgene Notes:
  • Addgene diagnostic digest
• A portion of this plasmid was derived from a plasmid made by: pEGFPC1-LC3 from Tamotsu Yoshimori at the National Institute of Genetics, Mishima, Japan.
• Articles Citing this Plasmid:
  • 91 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

LC3, a mammalian homologue of yeast Apg8p, is localized in autophagosome membranes after processing. Kabeya Y et al. (EMBO J. 2000 Nov 1. 19(21):5720-8.