pcDNA3-HA-PHLPP1 N-terminal truncation
(Plasmid #22404)

• Purpose: Expresses a HA tagged, N-terminal truncation of human PHLPP1 in mammalian cells
• Depositing Lab: Alexandra Newton
• Publication: Gao et al Mol Cell. 2005 Apr 1. 18(1):13-24.

Backbone

• Vector backbone: pcDNA3
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5446
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PHLPP1
• Alt name: SCOP
• Species: H. sapiens (human)
• Insert Size (bp): 3615
• Entrez Gene: PHLPP1 (a.k.a. PHLPP, PLEKHE1, PPM3A, SCOP)
• Tag / Fusion Protein:
  • HA (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: SP6

Resource Information

• Articles Citing this Plasmid:
  • 10 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that PHLPP1 N-terminal truncation was referred to as PHLPP1 in the original publication (Gao et al., 2005) and PHLPP1alpha in the subsequent publication (Brognard et al., 2007; PMID:
17386267).