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pcDNA3.1-P2X2-YFP
(Plasmid #22400)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 22400 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5600
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ATP-gated P2X channel 2
  • Alt name
    P2X2 receptors
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    2300
  • GenBank ID
    NM_053656
  • Entrez Gene
    P2rx2 (a.k.a. P2X2)
  • Tag / Fusion Protein
    • YFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HINDIII (not destroyed)
  • 3′ cloning site XHOI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer BGH-Rev
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    James Fisher and Ali Jones in Cambridge
  • Article Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1-P2X2-YFP was a gift from Henry Lester (Addgene plasmid # 22400 ; http://n2t.net/addgene:22400 ; RRID:Addgene_22400)

  • For your References section:

    An angstrom scale interaction between plasma membrane ATP-gated P2X2 and alpha4beta2 nicotinic channels measured with fluorescence resonance energy transfer and total internal reflection fluorescence microscopy. Khakh BS, Fisher JA, Nashmi R, Bowser DN, Lester HA. J Neurosci. 2005 Jul 20. 25(29):6911-20. 10.1523/JNEUROSCI.0561-05.2005 PubMed 16033901
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