pcDNA3.1-P2X2-YFP
(Plasmid #22400)

• Depositing Lab: Henry Lester
• Publication: Khakh et al J Neurosci. 2005 Jul 20. 25(29):6911-20.

Backbone

• Vector backbone: pcDNA3.1
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5600
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ATP-gated P2X channel 2
• Alt name: P2X2 receptors
• Species: R. norvegicus (rat)
• Insert Size (bp): 2300
• GenBank ID: NM_053656
• Entrez Gene: P2rx2 (a.k.a. P2X2)
• Tag / Fusion Protein:
  • YFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HINDIII (not destroyed)
• 3′ cloning site: XHOI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: BGH-Rev

Resource Information

• A portion of this plasmid was derived from a plasmid made by: James Fisher and Ali Jones in Cambridge
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry