Skip to main content
Addgene

PCRII-TOPO-ZFERV-ENV
(Plasmid #22399)

Print

Full plasmid sequence is not available for this item.

Loading...

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 22399 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    PCRII-TOPO
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 3950
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    DH5a
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ZFERV env gene
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    1993
  • GenBank ID
    AF503912
  • Entrez Gene
    env

Cloning Information

  • Cloning method TOPO Cloning
  • 5′ cloning site TOPO TA cloning (not destroyed)
  • 3′ cloning site TOPO TA cloning (not destroyed)
  • 5′ sequencing primer SP6
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

J. Virol. 78 (2), 899-911 (2004)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PCRII-TOPO-ZFERV-ENV was a gift from Lisa Steiner (Addgene plasmid # 22399 ; http://n2t.net/addgene:22399 ; RRID:Addgene_22399)
Related items:
Commonly requested with: