hIRmut-GFP
(Plasmid
#22397)
-
Depositing Lab
-
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 22397 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepEGFP-N2
-
Backbone manufacturerClontech
- Backbone size w/o insert (bp) 4700
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameIRmut
-
Alt nameIRL62P
-
SpeciesH. sapiens (human)
-
Insert Size (bp)4700
-
Mutationchanged Leu 62 to Proline
-
Tag
/ Fusion Protein
- GFP (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NheI (destroyed during cloning)
- 3′ cloning site ApaI (not destroyed)
- 5′ sequencing primer ACG CAA ATG GGC GGT AGG CG
- 3′ sequencing primer TCG CCG GAC ACG CTG AAC TTG TG (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
hIRmut-GFP was a gift from Joseph Bass (Addgene plasmid # 22397 ; http://n2t.net/addgene:22397 ; RRID:Addgene_22397) -
For your References section:
Calreticulin and Hsp90 stabilize the human insulin receptor and promote its mobility in the endoplasmic reticulum. Ramos RR, Swanson AJ, Bass J. Proc Natl Acad Sci U S A. 2007 Jun 19. 104(25):10470-5. 10.1073/pnas.0701114104 PubMed 17563366