Skip to main content
Addgene

pLV-H2B-iRFP670-p2a-mCerulean-Cdt1 (1-100)-IRES-puromycin
(Plasmid #223965)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 223965 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pLV-EF1a-IRES-puromycin
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    H2B-iRFP670-p2a-mCerulean-Cdt1 (1-100)
  • Alt name
    H2B
  • Alt name
    Cdt1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2462
  • Entrez Gene
    H2B
  • Entrez Gene
    CDT1 (a.k.a. DUP, RIS2)
  • Promoter EF-1a
  • Tags / Fusion Proteins
    • iRFP670
    • mCerulean

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer For H2B-iRFP670: ccatttcaggtgtcgtgaggatccgccaccATGCCAGAGCCAGCGAAGTCT; For p2a-mCerulean-Cdt1: caccaacgcctgtacaagggttccggagc
  • 3′ sequencing primer For H2B-iRFP670: cttgtacaggcgttggtggtgggcgg; For Cdt1: gcccgtcgactctagagcggccgccctcgaggaattcTTAtttctttatcttctggcccggagaaggg
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLV-H2B-iRFP670-p2a-mCerulean-Cdt1 (1-100)-IRES-puromycin was a gift from Hee Won Yang (Addgene plasmid # 223965 ; http://n2t.net/addgene:223965 ; RRID:Addgene_223965)

  • For your References section:

    Non-canonical pathway for Rb inactivation and external signaling coordinate cell-cycle entry without CDK4/6 activity. Zhang M, Kim S, Yang HW. Nat Commun. 2023 Nov 29;14(1):7847. doi: 10.1038/s41467-023-43716-y. 10.1038/s41467-023-43716-y PubMed 38030655
Related items: