pcDNA5-FRT-TOgw-C-mCherry
(Plasmid #223952)

• Purpose: (Empty Backbone) Gateway cloning of genes of interest with a C-terminal mCherry fusion; generation of stable cells with doxycycline inducible expression
• Depositing Labs: Karla Feijs, Roko Žaja
• Publication: Zaja lab plasmids (unpublished)

Backbone

• Vector backbone: pcDNA5-FRT-TOgw
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin
• Tag / Fusion Protein:
  • mCherry (C terminal on backbone)

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol and Ampicillin, 25 & 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): ccdB Survival
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: None
• Tag / Fusion Protein:
  • mCherry (C terminal on backbone)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry