pcDNA5-FRT-TOgw-C-mEYFP
(Plasmid
#223951)
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Purpose(Empty Backbone) Gateway cloning of genes of interest with a C-terminal mEYFP fusion; generation of stable cells with doxycycline inducible expression
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Depositing Labs
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 223951 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA5-FRT-TOgw
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Vector typeMammalian Expression
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Selectable markersHygromycin
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Tag
/ Fusion Protein
- mEYFP (C terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
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Tag
/ Fusion Protein
- mEYFP (C terminal on backbone)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA5-FRT-TOgw-C-mEYFP was a gift from Karla Feijs & Roko Žaja (Addgene plasmid # 223951 ; http://n2t.net/addgene:223951 ; RRID:Addgene_223951)
