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Addgene

RSV CREB M1
(Plasmid #22395)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 22395 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    RSV-SG
  • Backbone size w/o insert (bp) 3600
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    CREB
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    1200
  • Mutation
    Changed Serine 133 to Alanine
  • Entrez Gene
    Creb1 (a.k.a. Creb)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Xba 1 (destroyed during cloning)
  • 3′ cloning site Bal1 (destroyed during cloning)
  • 5′ sequencing primer RSV
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    RSV CREB M1 was a gift from Marc Montminy (Addgene plasmid # 22395 ; http://n2t.net/addgene:22395 ; RRID:Addgene_22395)

  • For your References section:

    Cyclic AMP stimulates somatostatin gene transcription by phosphorylation of CREB at serine 133. Gonzalez GA, Montminy MR. Cell. 1989 Nov 17. 59(4):675-80. 10.1016/0092-8674(89)90013-5 PubMed 2573431
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