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Addgene

pD(Nt)_His6
(Plasmid #223871)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 223871 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pD
  • Vector type
    Golden-Gate Donor

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Pir1 ; strain contains pir-116 mutation for plasmid maintenance at high copy
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    His6
  • Species
    Synthetic
  • Tag / Fusion Protein
    • 6xHis (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.06.17.599261 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pD(Nt)_His6 was a gift from Stephan Gruber (Addgene plasmid # 223871 ; http://n2t.net/addgene:223871 ; RRID:Addgene_223871)

  • For your References section:

    4G cloning: rapid gene assembly for expression of multisubunit protein complexes in diverse hosts. Taschner M, Dickinson JB, Roisné-Hamelin F, Gruber S. bioRxiv 2024.06.17.599261 10.1101/2024.06.17.599261
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