pMulti_A_ccdB
(Plasmid
#223851)
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Purpose(Empty Backbone) GEC-acceptor for 4G-cloning, designed for expression in E. coli (Ampicillin resistance)
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 223851 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET
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Vector typeBacterial Expression ; Golden-Gate Acceptor
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameNone
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Contains ccdB.
Please visit https://doi.org/10.1101/2024.06.17.599261 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMulti_A_ccdB was a gift from Stephan Gruber (Addgene plasmid # 223851 ; http://n2t.net/addgene:223851 ; RRID:Addgene_223851) -
For your References section:
4G cloning: rapid gene assembly for expression of multisubunit protein complexes in diverse hosts. Taschner M, Dickinson JB, Roisné-Hamelin F, Gruber S. bioRxiv 2024.06.17.599261 10.1101/2024.06.17.599261