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Addgene

AiP1999 - pAAV-AiE2255m-minBG-iCre(R297T)-BGHpA
(Plasmid #223843)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 223843 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pAAV
  • Total vector size (bp) 5016
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    iCre(R297T)
  • Species
    Synthetic
  • Insert Size (bp)
    1056
  • Mutation
    Argenine in aa297 was replaced with Threonine
  • Promoter minBG

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer GTTGCCAGCCATCTGTTGTTTGC
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AiP1999 - pAAV-AiE2255m-minBG-iCre(R297T)-BGHpA was a gift from Bosiljka Tasic (Addgene plasmid # 223843 ; http://n2t.net/addgene:223843 ; RRID:Addgene_223843)