AiP1999 - pAAV-AiE2255m-minBG-iCre(R297T)-BGHpA
(Plasmid #223843)

• Purpose: Enhancer-driven expression of the iCre(R297T) recombinase in thalamic PVT neurons
• Depositing Lab: Bosiljka Tasic
• Publication: Enhancer driven expression in thalamic populations (unpublished)

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Backbone

• Vector backbone: pAAV
• Total vector size (bp): 5016
• Vector type: AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: iCre(R297T)
• Species: Synthetic
• Insert Size (bp): 1056
• Mutation: Argenine in aa297 was replaced with Threonine
• Promoter: minBG

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: GTTGCCAGCCATCTGTTGTTTGC

Resource Information

• Supplemental Documents:
  • AiP1999.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry