pET50delCT_Vipp1_dL10Ala
(Plasmid
#223817)
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PurposeBacterial expression of Vipp1_dL10Ala with C-terminal 6x His-Tag and 3C cleavage site
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 223817 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET50
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameVipp1
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Alt nameIM30
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SpeciesSynechocystis sp. PCC6803
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Mutationreplacement of 10 aa in Hinge 2 with Ala
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Tag
/ Fusion Protein
- 6xHis (C terminal on insert)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET50delCT_Vipp1_dL10Ala was a gift from Carsten Sachse (Addgene plasmid # 223817 ; http://n2t.net/addgene:223817 ; RRID:Addgene_223817)