pRSET_SynPspA
(Plasmid
#223812)
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PurposeBacterial expression of SynPspA with N-terminal 10xHis-tag and EK cleavage site
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 223812 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepRSET
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSynPspA
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Alt namePspA
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SpeciesSynechocystis sp. PCC6803
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Tags
/ Fusion Proteins
- NusA (N terminal on backbone)
- 10xHis (N terminal on insert)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRSET_SynPspA was a gift from Carsten Sachse (Addgene plasmid # 223812 ; http://n2t.net/addgene:223812 ; RRID:Addgene_223812) -
For your References section:
PspA adopts an ESCRT-III-like fold and remodels bacterial membranes. Junglas B, Huber ST, Heidler T, Schlosser L, Mann D, Hennig R, Clarke M, Hellmann N, Schneider D, Sachse C. Cell. 2021 Jul 8;184(14):3674-3688.e18. doi: 10.1016/j.cell.2021.05.042. Epub 2021 Jun 23. 10.1016/j.cell.2021.05.042 PubMed 34166616