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Addgene

pcDNA3.1-Flag-Jarid2-polyA
(Plasmid #223811)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 223811 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1-polyA
  • Vector type
    Mammalian Expression ; In vitro transcription

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Jarid2
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Jarid2 (a.k.a. Jmj, jumonji)
  • Promoter CMV, T7
  • Tag / Fusion Protein
    • Flag (N terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1-Flag-Jarid2-polyA was a gift from Azusa Inoue (Addgene plasmid # 223811 ; http://n2t.net/addgene:223811 ; RRID:Addgene_223811)

  • For your References section:

    H3K27 dimethylation dynamics reveal stepwise establishment of facultative heterochromatin in early mouse embryos. Matsuwaka M, Kumon M, Inoue A. Nat Cell Biol. 2025 Jan;27(1):28-38. doi: 10.1038/s41556-024-01553-1. Epub 2024 Oct 31. 10.1038/s41556-024-01553-1 PubMed 39482357
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