SRS-MTBD(26/19) AB(monomer), wt
(Plasmid
#22381)
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 22381 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET42a
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Backbone manufacturerEMD
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameSeryl tRNA synthetase (SRS) – mouse cytoplasmic dynein microtubule binding domain (MTBD) fusion protein
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SpeciesM. musculus (mouse)
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Insert Size (bp)6646
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Tag
/ Fusion Protein
- myc (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer N/A (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
NB: There is a C-terminal His tag also present on the insert
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
SRS-MTBD(26/19) AB(monomer), wt was a gift from Ron Vale (Addgene plasmid # 22381 ; http://n2t.net/addgene:22381 ; RRID:Addgene_22381) -
For your References section:
The affinity of the dynein microtubule-binding domain is modulated by the conformation of its coiled-coil stalk. Gibbons IR, Garbarino JE, Tan CE, Reck-Peterson SL, Vale RD, Carter AP. J Biol Chem. 2005 Jun 24. 280(25):23960-5. 10.1074/jbc.M501636200 PubMed 15826937
