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pGEX4T1_GST-EGFP-ATG13_IDR_191-517aa_(M196-H214 11xA)
(Plasmid #223779)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 223779 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEX4T-1
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    ATG13 IDR
  • Species
    H. sapiens (human)
  • Mutation
    expresses aa 191-517, with M196A S197A R199A G202A T204A P205A I207A M208A I210A D213A H214A disrupts WIPI binding
  • Entrez Gene
    ATG13 (a.k.a. KIAA0652, PARATARG8)
  • Tag / Fusion Protein
    • GST-EGFP (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Internal #: SMc2450 . Please visit https://doi.org/10.1101/2024.08.28.609967 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX4T1_GST-EGFP-ATG13_IDR_191-517aa_(M196-H214 11xA) was a gift from Sascha Martens (Addgene plasmid # 223779 ; http://n2t.net/addgene:223779 ; RRID:Addgene_223779)

  • For your References section:

    Reconstitution of BNIP3/NIX-mitophagy initiation reveals hierarchical flexibility of the autophagy machinery. Adriaenssens E, Schaar S, Cook ASI, Stuke JFM, Sawa-Makarska J, Nguyen TN, Ren X, Schuschnig M, Romanov J, Khuu G, Lazarou M, Hummer G, Hurley JH, Martens S. bioRxiv 2024.08.28.609967 10.1101/2024.08.28.609967
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