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pFastBac-Dual_BNIP3(1-158aa)-THROMBIN-GST (W18A/L21A; deltaLIR)
(Plasmid #223778)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 223778 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFBD
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    BNIP3 soluble part
  • Species
    H. sapiens (human)
  • Mutation
    W18A/L21A; deletion of LIR
  • Entrez Gene
    BNIP3 (a.k.a. HABON, NIP3)
  • Tag / Fusion Protein
    • Thrombin cleavage-GST (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Internal #: SMc2445 . Please visit https://doi.org/10.1101/2024.08.28.609967 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFastBac-Dual_BNIP3(1-158aa)-THROMBIN-GST (W18A/L21A; deltaLIR) was a gift from Sascha Martens (Addgene plasmid # 223778 ; http://n2t.net/addgene:223778 ; RRID:Addgene_223778)

  • For your References section:

    Reconstitution of BNIP3/NIX-mediated autophagy reveals two pathways and hierarchical flexibility of the initiation machinery. Adriaenssens E, Schaar S, Cook ASI, Stuke JFM, Sawa-Makarska J, Nguyen TN, Ren X, Schuschnig M, Romanov J, Khuu G, Lazarou M, Hummer G, Hurley JH, Martens S. bioRxiv [Preprint]. 2024 Aug 28:2024.08.28.609967. doi: 10.1101/2024.08.28.609967. 10.1101/2024.08.28.609967 PubMed 39253418
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