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Addgene

pSRK51
(Plasmid #223722)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 223722 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET-28b
  • Total vector size (bp) 6411
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Express in BL21-RIL
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    TEV protease
  • Species
    tobacco etch virus
  • Mutation
    L56V/S135G/S219V
  • Promoter T7
  • Tag / Fusion Protein
    • NT* (self cleaving) (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSRK51 was a gift from Sreejith Raran-Kurussi (Addgene plasmid # 223722 ; http://n2t.net/addgene:223722 ; RRID:Addgene_223722)

  • For your References section:

    Advancing large-scale production of TEV protease through an innovative NT* tag-based fusion construct. Parida PP, Saraswathi D, Mopidevi SMV, Raran-Kurussi S. Curr Res Struct Biol. 2023 Sep 30;6:100106. doi: 10.1016/j.crstbi.2023.100106. eCollection 2023. 10.1016/j.crstbi.2023.100106 PubMed 37822550
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