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pCAGGS-Twin-strep-TEV-DGKδ2-DSAMD
(Plasmid #223721)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 223721 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCAGGS-C-TEV-Twin-Strep
  • Backbone manufacturer
    Chiaki Murakami
  • Backbone size w/o insert (bp) 4860
  • Total vector size (bp) 8322
  • Modifications to backbone
    TEV, Twin-Strep-tag, and stop codon (TGA) were subcloned into the XhoI/BglII site of pCAGGS.
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    DGKD
  • Alt name
    Diacylglycerol kinase delta 2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3432
  • Mutation
    deleted sterile alpha motif domain (deleted amino acids 1144–1214)
  • GenBank ID
    NM_152879.3
  • Entrez Gene
    DGKD (a.k.a. DGK-delta, DGKdelta, dgkd-2)
  • Promoter chicken β-actin promoter
  • Tags / Fusion Proteins
    • Twin-Strep-tag (N terminal on backbone)
    • TEV cleavable site (N terminal on backbone)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer TTCGGCTTCTGGCGTGTGACC
  • 3′ sequencing primer TATTAGCCAGAAGTCAGATGCTCAAGG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The insert can be removed using the restriction enzyme, BglII.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAGGS-Twin-strep-TEV-DGKδ2-DSAMD was a gift from Chiaki Murakami (Addgene plasmid # 223721 ; http://n2t.net/addgene:223721 ; RRID:Addgene_223721)
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