pGH1110
(Plasmid
#223682)
-
Purpose(Empty Backbone) U6-CloningSite-Ef1a-Cas9-NLS-Flag-T2A-EGFP-P2A-BlastR
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 223682 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepLentiCRISPR_v2
-
Modifications to backboneAdded Cas9-T2A-GFP-P2A-BlastR instead of puro resistance
-
Vector typeMammalian Expression, Lentiviral, CRISPR
-
Selectable markersBlasticidin
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)NEB Stable
-
Copy numberHigh Copy
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pGH1110 was a gift from Gaelen Hess (Addgene plasmid # 223682 ; http://n2t.net/addgene:223682 ; RRID:Addgene_223682) -
For your References section:
A High-Throughput Screen for Antiproliferative Peptides in Mammalian Cells Identifies Key Transcription Factor Families. Liila-Fogarty SM, Boyum GE, Schwabe CL, Hess GT. ACS Synth Biol. 2024 Oct 19. doi: 10.1021/acssynbio.4c00337. 10.1021/acssynbio.4c00337 PubMed 39425622