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Addgene

PB-GFP11x7-HygR
(Plasmid #223676)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 223676 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    PiggyBac
  • Total vector size (bp) 8292
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GFP11x7
  • Alt name
    GFP
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    887
  • GenBank ID
  • Promoter CMV
  • Tag / Fusion Protein
    • GFP11 (C terminal on insert)

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer gacattgattattgactagttattaatagtaatcaattacggggtcattagttcatagcccatatatggagttccgcgttacataacttacggtaaatggcccgcctg
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PB-GFP11x7-HygR was a gift from Juan Melero-Martin (Addgene plasmid # 223676 ; http://n2t.net/addgene:223676 ; RRID:Addgene_223676)

  • For your References section:

    Mitochondrial transfer mediates endothelial cell engraftment through mitophagy. Lin RZ, Im GB, Luo AC, Zhu Y, Hong X, Neumeyer J, Tang HW, Perrimon N, Melero-Martin JM. Nature. 2024 May;629(8012):660-668. doi: 10.1038/s41586-024-07340-0. Epub 2024 May 1. 10.1038/s41586-024-07340-0 PubMed 38693258
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