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Addgene

pNHA8
(Plasmid #223650)

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Item Catalog # Description Quantity Price (USD)
Plasmid 223650 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pQE80L
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    BccP
  • Species
    Shigella flexneri M90T
  • Promoter LacI
  • Tag / Fusion Protein
    • MBP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site PstI (not destroyed)
  • 5′ sequencing primer TGC TTT GTG AGC GGA TAA C
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pNHA8 was a gift from François-Xavier Campbell-Valois (Addgene plasmid # 223650 ; http://n2t.net/addgene:223650 ; RRID:Addgene_223650)

  • For your References section:

    The promiscuous biotin ligase TurboID reveals the proxisome of the T3SS chaperone IpgC in Shigella flexneri. Haidar-Ahmad N, Tomaro K, Lavallée-Adam M, Campbell-Valois F. 0.. mSphere 0:e00553-24 10.1128/msphere.00553-24
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